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膜通道蛋白与心血管疾病研究室
发布日期:2015-08-27浏览次数: 字号:[ ]

 

 

 

 
杨宝学 博士
主任

Baoxue Yang, M.D., Ph.D.
Phone:010-82805622
Cell: 13439310105
Fax:  010-82805622
E-mail:baoxue@bjmu.edu.cn

 

 
教育经历
Education
1984         白求恩医科大学医学系   医学学士
              Department of Medicine, Norman Bethune University of Medical Sciences, Changchun, China, M.D.
1987         白求恩医科大学医学遗传学教研室   医学遗传学硕士
              Department of Medical Genetics, Norman Bethune University of Medical Sciences, Changchun, China, M.S.
1993         白求恩医科大学免疫学教研室   免疫学博士
              Department of Immunology, Norman Bethune University of Medical Sciences, Changchun, China, Ph.D.
 
 
 
工作经历
Professional Experience
 
2007-present    北京大学基础医学院药理学系 教授, 博士生导师, 系副主任
                                 Department of Pharmacology, School of Basic Medical Sciences, Peking University, Beijing, China
2003-2009               美国加州大学旧金山分校医学系, 助理教授
                                 Assistant professor, Department of medicine, University of California, San Francisco, USA
1997-2003       美国加州大学旧金山分校医学系, 助理研究员
                                 Assistant Molecular Biologist, Department of medicine, University of California, San Francisco, USA
1994-1997       美国加州大学旧金山分校心血管研究所 博士后
                Postdoctoral Fellow, Cardiovascular Research Institute, University of California, San Francisco, USA
1993-1994       白求恩医科大学免疫学教研室 副教授
                Associate Professor, Department of Immunology,Norman Bethune University of Medical Sciences, Changchun, China
1997-2000       白求恩医科大学医学遗传学教研室 讲师  
                Lecturer, Department of Medical Genetics, Norman Bethune University of Medical Sciences, Changchun, China
 
 
研究室成员
周虹 讲师
雷天落 讲师
高晋生 博士生 07级
李欣 博士生 08 级
李飞 博士生 09 级
周澧 博士生 04 级 (八年制)
陈霁辉 博士生 05 级 (八年制)
孙颖 博士生 06 级 (八年制)
 
 
 
研究室概述:
本研究室致力于心血管疾病相关药物新靶点探索、新型利尿剂研发、膜通道蛋白生理学与药理学研究、多囊肾发病机制和治疗研究。目前已建立多囊肾药物开发研究平台、水通道和尿素通道抑制剂筛选平台和膜通道蛋白生理学与药理学研究平台。具有良好的分子生物学、生理学和药理学研究条件。拥有多囊肾小鼠模型、水通道蛋白基因敲除小鼠模型和尿素通道蛋白基因敲除小鼠模型。已与美国Verkman 研究小组、美国Sands研究小组、法国Bankir研究小组和吉林大学病理生理学教研室进行合作研究。本实验室并不局限于已建立的研究框架,愿意同其他研究单位开展联合研究项目。
 
Research Description:
The main interest of the laboratory is focused on drug targets on cardiovascular diseases, new diuretics discovery, physiology and pharmacology of membrane channel proteins, and mechanism and therapy of polycystic kidney disease. The platforms for studying polycystic kidney disease, screening inhibitors of aquaporins and urea transporters and investigating physiology and pharmacology of membrane channel proteins have been established. There is good research condition for the research work on molecular biology, physiology and pharmacology. We have polycystic kidney disease mouse model, aquaporin gene knockout mouse models and urea transporter gene knockout mouse models. We collaborate with Verkman’s group (UCSF, USA), Sands’ group (Emory University, USA), Bankir’s group (Inserm, France) and Department of Pathophysiology, Jilin University on several joint projects. We would like to have collaboration with the other groups for joint projects in various research fields.
 
 
 
Selected Publications:
 
 
1.     Yu H., Y.Meng, L.Wang, X.Jin, L.Gao, L.Zhou, K.Ji, Y.Li, L.Zhao, G.Chen, X.Zhao, and B.Yang*. (2009) Differential Protein Expression in Heart in UT-B Null Mice with Cardiac Conduction Defects. Proteomics 9:504-511. [IF 5.479]
 
2.     Yang B.*, D. Zhao, and A.S. Verkman. (2009) Hsp90 inhibitor partially corrects nephrogenic diabetes insipidus in a conditional knock-in mouse model of aquaporin-2 mutation. FASEB J. 23:503-512. [IF 6.791]
 
3.     Yang B., N.D. Sonawane, D. Zhao, S. Somlo, and A.S. Verkman. (2008) Small-molecule CFTR inhibitors slow cyst growth in polycystic kidney disease. J Am Soc Nephrol 19:1300-1310. [IF 7.111]
 
4.     Yang B., Z. Zador, and A.S. Verkman. (2008) Glial cell aquaporin-4 overexpression in transgenic mice accelerates cytotoxic brain swelling. J Biol Chem 283:15280-15286. [IF 5.581]
 
5.     Yang B., H. Zhang, and A.S. Verkman. (2008) Lack of aquaporin-4 water transport inhibition by antiepileptics and arylsulfonamides. Bioorg Med Chem 16:7489-7493. [IF 2.624]
 
 
 
8.     Yang B .*, D. Zhao, L. Qian, and A.S. Verkman. (2006) Mouse model of inducible nephrogenic diabetes insipidus produced by floxed aquaporin-2 gene deleltion. Am J Physiol Renal Physiol291:F465-F472. [IF 4.199]
 
9.     Zhao D., L. Bankir, L. Qian, D. Yang, and B. Yang*. (2006) Urea and urine concentrating ability in mice lacking AQP1 and AQP3. Am J Physiol Renal Physiol 291:F429-F438. [IF 4.199]
 
10.    Yang B.*, D. Zhao, S. Eugene, and A.S. Verkman. (2006) Evidence from knockout mice against physiologically significant aquaporin-8 facilitated ammonia transport. Am J Physiol cell Physiol 291:C417-C423. [IF 4.334]
 
11.    Yang B., D. Zhao, and A.S. Verkman. (2006) Evidence against functionally significant aquaporin expression in mitochondria. J Biol Chem. 281:16202-16206. [IF 5.808]
 
12.    Yang B.*, J.K. Kim, and A.S. Verkman. (2006) Comparative efficacy of HgCl(2) with candidate aquaporin-1 inhibitors DMSO, gold, TEA(+) and acetazolamide. FEBS Lett 580:6679-6684. [IF 3.372]
 
13.    Meng Y., X. Zhou, Y. Li, D. Zhao, S. Liang, X. Zhao, and B. Yang*. (2005) A novel mutation at the JK locus causing Jk null phenotype in a Chinese family. Sci China C Life Sci 48:636-640. [IF 0.482]
 
14.    Yang B., Y. Song, D. Zhao, and A.S. Verkman. (2005) Phenotype analysis of aquaporin-8 null mice. Am J Physiol cell Physiol 288:C1161-C1170. [IF 3.942]
 
15.    Klein J.D., J.M. Sands, L. Qian, X. Wang, and B. Yang*. (2004) Upregulation of urea transporter UT-A2 and water channels AQP2 and AQP3 in mice lacking urea transporter UT-B. J Am Soc Nephrol 15:1161-1167. [IF 6.644]
 
16.    Bankir L., K. Chen, and B. Yang*. (2004) Lack of UT-B in vasa recta and red blood cells prevents urea-induced improvement of urinary concentrating ability. Am J Physiol Renal Physiol 286:F144-F151. [IF 4.354]
 
17.    Yang B., L. Bankir, A. Gillepsie, C. J. Epstein, and A. S. Verkman. (2002) Urea-selective concentrating defect in transgenic mice lacking urea transporter UT-B. J BIol Chem 277:10633-10637. [IF 6.696]
 
18.    Yang B., and A. S. Verkman. (2002) Analysis of double knokout mice lacking aquaporin-1 and urea transporter UT-B: Evidence for UT-B facilitated water transport in erythrocytes. J Biol Chem 277:36782-36786. [IF 6.696]
 
19.    Yang B., T. Ma, and A. S. Verkman. (2001) Erythrocyte water permeability and renal function in double knockout mice lacking aquaporin-1 and aquaporin-3. J Biol Chem 276:624-628. [IF 7.258]
 
20.    Yang B., A. Gillespie, E. J. Carlson, C. J. Epstein, and A. S. Verkman. (2001) Neonatal mortality in an aquaporin-2 knock-in mouse model of recessive nephrogenic diabetes insipidus. J Biol Chem 276:2775-2779. [IF 7.258]
 
21.    Yang B., J. M. Verbavatz, Y. Song, L. Vetrivel, G. Manley, W. M. Kao, T. Ma, and A. S. Verkman. (2000) Skeletal muscle function and water permeability in aquaporin-4 deficient mice. Am J Physiol Cell Physiol 278:C1108-C1115. [IF 4.086]
 
22.    Yang B., T. Ma, J. Y. Dong, and A. S. Verkman. (2000) Partial correction of the urinary concentrating defect in aquaporin-1 null mice by adenovirus-mediated gene delivery. Hum Gene Ther 11:567-575. [IF 6.796]
 
23.    Yang B., N. Fukuda, A. van Hoek, M. A. Matthay, T. Ma, and A. S. Verkman. (2000) Carbon dioxide permeability of aquaporin-1 measured in erythrocytes and lung of aquaporin-1 null mice and in reconstituted proteoliposomes. J Biol Chem 275:2686-2692. [IF 7.368]
 
24.    Yang B., T. Ma, Z. Xu, and A. S. Verkman. (1999) cDNA and genomic cloning of mouse aquaporin-2: functional analysis of an orthologous mutant causing nephrogenic diabetes insipidus. Genomics 57:79-83. [IF 3.386]
 
25.    Yang B., H. G. Folkesson, J. Yang, M. A. Matthay, T. Ma, and A. S. Verkman. (1999) Reduced osmotic water permeability of the peritoneal barrier in aquaporin-1 knockout mice. Am J Physiol 276:C76-C81. [IF 3.485]
 
26.    Yang B., and A. S. Verkman. (1998) Urea transporter UT3 functions as an efficient water channel. Direct evidence for a common water/urea pathway. J Biol Chem 273:9369-9372. [IF 7.199]
 
27.   Yang B., and A. S. Verkman. (1997) Water and glycerol permeabilities of aquaporins 1-5 and MIP determined quantitatively by expression of epitope-tagged constructs in Xenopus oocytes. J Biol Chem 272:16140-16146. [IF 6.963]           
 
28.    Yang B., A. N. van Hoek, and A. S. Verkman. (1997) Very high single channel water permeability of aquaporin-4 in baculovirus-infected insect cells and liposomes reconstituted with purified aquaporin-4. Biochemistry 36:7625-7632. [IF 4.572]
 
29.   Yang B., D. Brown, and A. S. Verkman. (1996) The mercurial insensitive water channel (AQP-4) forms orthogonal arrays in stably transfected Chinese hamster ovary cells. J Biol Chem 271:4577-4580. [IF 7.452]
 
30.   Yang B., T. Ma, and A. S. Verkman. (1995) cDNA cloning, gene organization, and chromosomal localization of a human
mercurial insensitive water channel. Evidence for distinct transcriptional units. J Biol Chem 270:22907-22913. [IF 7.667]

 




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